Lipoxygenase inhibitory activity evaluation of Achillea biebersteinii Afan. by activity-guided fractionation
JOURNAL OF ETHNOPHARMACOLOGY, cilt.357, ss.120900, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 357
- Basım Tarihi: 2026
- Doi Numarası: 10.1016/j.jep.2025.120900
- Dergi Adı: JOURNAL OF ETHNOPHARMACOLOGY
- Derginin Tarandığı İndeksler: Academic Search Ultimate (EBSCO), Scopus, Science Citation Index Expanded (SCI-EXPANDED), BIOSIS, CINAHL, EMBASE, Index Islamicus, MEDLINE
- Sayfa Sayıları: ss.120900
- Anadolu Üniversitesi Adresli: Evet
Özet
Ethnopharmacological relevance
Achillea biebersteinii Afan. is traditionally utilized as folk medicine for a broad range of therapeutic applications, especially for promoting the maturation of abscesses, wound healing; against inflammation, and rheumatism in Türkiye.
Aim of the study
The anti-inflammatory potential of A. biebersteinii was evaluated through activity-guided fractionation (AGF) targeting lipoxygenase (15-LOX) inhibition.
Materials and methods
Different chromatographic techniques were used for the AGF of the ethyl acetate extract of A. biebersteinii aerial parts. The in vitro 15-LOX inhibitory activity evaluation was performed to address the anti-inflammatory activity. The structures of purified compounds from the fractions were confirmed by LC-HRMS, 1H NMR, and 13C NMR analyses, respectively.
Results
The fractionation and isolation process culminated in the identification of three key flavonoids namely; patulitrin, axillarin, quercetagetin-7-O-β-glucopyranoside, and 4,5-dicaffeoylquinic acid, which showed statistically remarkable 15-LOX inhibitory activity with inhibition rates of 82.27 %, 96.81 %, 84.65 % and 77.47 %, respectively. Two flavonoids were isolated by using the AGF method, where quinic acid was spotted to have significant 15-LOX inhibitory activity.
Conclusion
These findings support the future therapeutic potential of A. biebersteinii as a natural anti-inflammatory source.