The aim of the research was to investigate the chemical composition of essential oils and volatiles from two species of Artemisia glabella and Artemisia rupestris growing in Kazakhstan. Two different techniques, the conventional hydrodistillation (HD) and modern fast micro-steam distillation-solid-phase microextraction (MSD-SPME) have been used to obtain the volatiles. Chemical profiles of the volatiles were comparatively analyzed with GC-FID/MS techniques. The yields of essential oils from A. glabella and A. rupestris obtained by a hydrodistillation were 0.2% and 0.1%, respectively. The major components of A. rupestris essential oil were myrcene (9.5%), β-elemene (5.4%) and capric acid (5.1%). The oil of A. glabella was found to be rich with 1,8-cineole (12.2%), cumin aldehyde (9.4%), α-terpineol (5.7%) and borneol (5.2%).