SYNTHESIS-STUTTGART, vol.48, no.3, pp.365-378, 2016 (SCI-Expanded)
A microwave-assisted procedure for labeling amines, amino acids, and peptides with 2,2'-azopyridines (2,2'-AzPy) is described using the corresponding 2,2'-azopyridine-diacylbenzotriazoles. The efficiency of the procedure is proven by the generation of three constitutionally isomeric sets of 2,2'-AzPy-X,X'-labeled amino conjugates (where X = 4, 5, 6) including amines, amino acids, and peptides. Microwave- assisted synthesis conditions provide good to excellent yields in less than 15 minutes with retention of original chirality. A trans-to-cis isomerization of the 2,2'-azopyridine-labeled amino conjugate upon laser irradiation at 325 nm is visualized with UV and NMR spectroscopy.