Effects of integrin-linked kinase on protein kinase b, glycogen synthase kinase-3 beta, and beta-catenin molecules in ovarian cancer cells

Sarı Kılıçaslan S. M., Incesu Z.

IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol.24, no.11, pp.1500-1508, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 24 Issue: 11
  • Publication Date: 2021
  • Doi Number: 10.22038/ijbms.2021.58716.13042
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, EMBASE, Index Islamicus, Veterinary Science Database, Directory of Open Access Journals
  • Page Numbers: pp.1500-1508
  • Keywords: Beta-catenin, Epithelial ovarian cancer, Fibronectin, GSK-3beta, Integrin, EXPRESSION INCREASES, ILK, ADHESION, EPIDEMIOLOGY, METASTASIS
  • Anadolu University Affiliated: Yes


Objective(s): This study examines the impact of integrin-linked kinase (ILK), protein kinase B (AKT), glycogen synthase kinase-3 beta (GSK-3 beta), and beta-catenin signal molecules in SKOV-3 ovarian cancer cells adhered to fibronectin. Materials and Methods: Expression levels of alpha 4, alpha v, beta 1, and beta 6 integrin subunits known as the fibronectin ligand were investigated with the flow cytometry technique. The effects of ILK, AKT, GSK3 beta, and beta-catenin on the binding of SKOV-3 cells to fibronectin were examined by using the Real-Time Cellular Analysis (RTCA) method. Additionally, the interaction of these proteins was investigated by using Western blot analysis. Results: The results show that the expression levels of integrin subunits were ranked as alpha v (67.8%), followed by alpha 4 (48.55%), beta 6 (32.05%), and beta 1 (31%) on SKOV-3 cells. RTCA results showed that ILK (10 mu M Cpd22), GSK-3 beta (50 mu M GSK-3 beta inhibitor-XI), AKT (35 mu M FPA 124), and beta-catenin (50 mu M cardamonin) inhibitors decreased significantly (P<0.01) binding to fibronectin at 24 hr. Western studies in SKOV-3 cells adhered to fibronectin have shown that in inhibition of ILK, AKT expression was strongly inhibited, whereas, in the inhibition of AKT, ILK expression was strongly inhibited. Furthermore, the expression of beta-catenin is partially reduced in inhibition of these two molecules. In beta-catenin inhibition, AKT and ILK expressions are also strongly inhibited. Conclusion: ILK, AKT, GSK-3 beta, and beta-catenin were found to be fundamental molecules in binding of SKOV-3 cells to fibronectin. ILK and AKT affect strongly the level of expression of each other, and both also affect the signal path of beta-catenin.