Nano-hemoglobin film based sextet state biomemory device by cross-linked photosensitive hapten monomer

GÜZEL R., ERSÖZ A., ZİYADANOĞULLARI R., Say R.

TALANTA, vol.176, pp.85-91, 2018 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 176
  • Publication Date: 2018
  • Doi Number: 10.1016/j.talanta.2017.08.014
  • Journal Name: TALANTA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.85-91
  • Keywords: Biomemory device, Hemoglobine, Nanobiochip, Chronoamperometry, Photosensitive monomer, DIRECT ELECTROCHEMISTRY, CARBON NANOTUBE, ELECTRODE, DNA, ELECTROCATALYSIS, BEHAVIOR, WATER, ASSAY
  • Anadolu University Affiliated: Yes

Abstract

In this study, a biomemory device, consisting of hemoglobin (Hb) cross-linked by MACys-Ru(bipyr)(2)-MACys) photosensitive monomer cross-linkers, which have memory effect through both Ru3+/2+ in hapten monomer and Fe3+/2+ in redox active center of Hb through multi-charge transfer mechanism, has been improved. Cyclic voltammetry (CV) has been used to determine the redox property of the Hb cross-linked MACys-Ru(bipyr)(2)-MACys) hapten. Three memory functions, writing, reading and erasing of the fabricated biomemory device, have been accomplished by chronoamperometry (CA) and open-circuit potential amperometry (OCPA). The reliability and repeatability of the biodevice consisting of the p(Hb-co-MACys-Ru(bipyr)(2)-MACys) sextet state bio-memory layer have been analysed. The Hb film based biodevice on gold electrodes has shown >= 2 months the retention time and switched until 10(6) times continuous cycling without degradation in efficiency. Other hand, the topography of p(Hb-co-MACys-Ru(bipyr)(2)-MACys) layer on the gold surface has investigated by scanning electron microscopy (SEM) and EDX data.