RuBisCO nano enzyme for mimicking CO(2)conversion system in plants

Bicen Unluer O., Say R., ERSÖZ A.

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, cilt.68, sa.2, ss.392-403, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 68 Sayı: 2
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1002/bab.1937
  • Dergi Adı: BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Applied Science & Technology Source, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Compendex, Computer & Applied Sciences, EMBASE, Environment Index, Food Science & Technology Abstracts, INSPEC, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.392-403
  • Anahtar Kelimeler: RuBisCO enzyme, ANADOLUCA, enzymatic CO(2)conversion, GENE-EXPRESSION, CARBON, CO2, NANOPARTICLES, CONVERSION, CATALYSIS, ASSAY
  • Anadolu Üniversitesi Adresli: Hayır

Özet

In this study, carbon dioxide (CO2) capture and conversion systems based on the combination of biomimetic systems with nano enzymes have been developed. The effectiveness of the developed system has been investigated toward CO(2)conversion. For this aim, nano ribulose bisphosphate carboxylase/oxygenase (RuBisCO) enzyme that plays role in the Calvin cycle in photosynthesis has been synthesized in 93 nm size according to AmiNoAcid (monomer) Decorated and Light Underpinning Conjugation Approach (ANADOLUCA) method. Enzymatic activity of synthesized nano RuBisCO enzyme has been spectrophotometrically determined by the formation of 3-phosphoglycerate (3-PGA) at the end of the reaction between CO(2)andd-ribulose-1,5 biphosphate with the catalysis of RuBisCO enzyme at 340 nm. The effect of substrate concentration, pH, temperature, and Mg(2+)ion concentration on the conversion reaction have investigated comparatively with nano and free RuBisCO enzyme. Besides this, the reusability feature of synthesized nano RuBisCO enzyme in conversion of CO(2)reaction is indicated. When all data were evaluated, it has been seen that the nano RuBisCO enzyme is effective on the conversion of CO(2)into 3-PGA and can be used for CO(2)capture and conversion systems repeatedly without any deformation in its structure.