A validated HPLC method with fluorescence detection for the determination of droperidol in pharmaceutical tablets, human serum, and human milk

Yeniceli D., Dogrukol-Ak D., Tuncel M.

CHROMATOGRAPHIA, 2007 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1365/s10337-007-0255-1
  • Dergi Adı: CHROMATOGRAPHIA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: column liquid chromatography, fluorimetric detection, pharmaceuticals in human serum and human milk, Droperidol, PERFORMANCE LIQUID-CHROMATOGRAPHY, SPECTROPHOTOMETRIC DETERMINATION, BUTYROPHENONES, HALOPERIDOL, DEGRADATION, PLASMA, DRUGS, BLOOD
  • Anadolu Üniversitesi Adresli: Evet

Özet

A sensitive and simple HPLC method with fluorimetric detection has been developed for determination of droperidol in pharmaceutical tablets, human serum, and human milk. Chromatography was performed on a 100 mm x 3 mm i.d. C-18 column with methanol-water, 30:70 (v/v), PH 3.5, as mobile phase at a flow-rate of 0.8 mL min(-1). The injection volume was 5 mu l. and detection was by monitoring emission at 324 nm after excitation at 283 nm. Droperidol and p-hydroxybenzoic acid (internal standard) eluted after 5.3 and 6.1 min, respectively. The method was validated over the concentration range 1.14 X 10(-7) to 9.12 x 10(-6) M. Selectivity was good and the limits of detection and quantitation of the method were approximately 3.54 x 10(-8) and 1.07 x 10(-7) M, respectively, corresponding to 13 and 40 ng mL(-1). The applicability of the method to determination of droperidol in pharmaceuticals, human serum, and human milk was demonstrated.