Akademik Veri Yönetim Sistemi
11th International Mediterranean Symposium on Medicinal and Aromatic Plants, Rimini, İtalya, 6 - 08 Mayıs 2025, ss.115, (Özet Bildiri)
BIOLOGICAL ACTIVITY STUDIES AND ESSENTIAL OIL ANALYSIS ON THYMUS CILICICUS
Fatmanur Tunç1, Pervin Soyer2, Yavuz Bülent Köse3, Mine Kürkçüoğlu4
1 Department of Pharmaceutical Botany, Faculty of Pharmacy, Anadolu University, 26470, Eskişehir, Türkiye
2 Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Anadolu University, Eskişehir, Türkiye
3 Department of Pharmaceutical Botany, Faculty of Pharmacy, Anadolu University, 26470, Eskişehir, Türkiye
4 Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, 26470, Eskişehir, Türkiye
E-mail: fatmanurt@anadolu.edu.tr, mkurkcuo@anadolu.edu.tr, pervinsoyer@anadolu.edu.tr, ybkose@anadolu.edu.tr
Thymus L., an important genus among aromatic plants in the Lamiaceae family, is represented by 60 taxa and 40 species in Türkiye. 18 of these species are endemic [1,2]. Infusions and decoctions of the leaves and flowering parts of Thymus have been used traditionally to prevention and treatment of numerous digestive and respiratory disorders including colds, flu, indigestion, nausea, and dysentery [3]. In this study, the antioxidant and antimicrobial properties and total phenolic content of 70% ethanol and ethyl acetate extracts of Thymus cilicicus Boiss. & Balansa were investigated. The chemical composition of the essential oil was analyzed. Antioxidant activity was evaluated using the DPPH method; 70% ethanol extract showed 96% inhibition, while ethyl acetate extract exhibited 36%. Total phenol content was determined using the Folin-Ciocalteu method, revealing that the 70% ethanol extract contained a higher phenolic content (113.6±0.103 mg GAE/g) compared to the ethyl acetate extract (23.23±0.04 mg GAE/g). Antimicrobial activity was determined by the microdilution method and MIC value was calculated against microorganisms. 70% ethanol extracts were more effective than ethyl acetate extracts. Antimicrobial activity was observed against standard bacterial species (Bacillus subtilis NRRL B478, Escherichia coli ATCC 35218, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 29213) at a concentration of 1040 μg/mL and against yeast species (Candida albicans ATCC 90028, Candida krusei ATCC 6258) at a concentration of 4160 μg/mL. Ethyl acetate extracts were effective at concentrations of 1040-4160 μg/mL. As a result of the analysis performed using the GC/GC-MS method, 55 compounds representing 98.9% of the essential oil were identified. The main compounds are α-Terpineol 18.5% and Geraniol 13.7%. As a result, the antioxidant and antimicrobial activity, and total phenol content of 70% ethanol extract were higher. In this study on T. cilicicus, an aromatic and medicinal plant collected from Eskişehir province, contributes valuable information to the existing literature.
Key Words: Thymus cilicicus, essential oil analysis, antioxidant activity, antimicrobial activity, total phenolic content
References
[1] https://www.bizimbitkiler.org.tr/v2/hiyerarsi.php?c=Thymus [2] Baser, K. (2002). Aromatic biodiversity among the flowering plant taxa of Turkey. Pure and Applied Chemistry, 74(4), 527-545. https://doi.org/10.1351/pac200274040527 [3] Li, X., He, T., Wang, X., Shen, M., Yan, X., Fan, S., ... & She, G. (2019). Traditional uses, chemical constituents and biological activities of plants from the genus Thymus. Chemistry & biodiversity, 16(9), e1900254.