Molecular recognition based iron removal from human plasma with imprinted membranes

Yavuz H., Andac M., UZUN L., Say R., Denizli A.

INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS, vol.29, no.9, pp.900-911, 2006 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 29 Issue: 9
  • Publication Date: 2006
  • Doi Number: 10.1177/039139880602900911
  • Journal Name: INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.900-911
  • Keywords: molecular imprinting, molecular recognition, iron removal, metal detoxification, affinity binding, THERAPEUTIC AFFINITY ADSORPTION, SOLID-PHASE EXTRACTION, CHELATION-THERAPY, ANTIBODY REMOVAL, CADMIUM REMOVAL, BEADS, PRECONCENTRATION, MICROSPHERES, DEFEROXAMINE, MICROBEADS
  • Anadolu University Affiliated: No

Abstract

The aim of this study is to prepare ion-imprinted poly(2-hydroxyethyl methacrylate) ( HEMA) based membranes which can be used for the selective removal of Fe3+ ions from Fe(3+)overdosed human plasma. N-methacryloyl-(L)-glutamic acid (MAGA) was chosen as the ion-complexing monomer. In the first step, Fe3+ was complexed with MAGA and then, the Fe3+-imprinted poly(HEMA-MAGA) membranes were prepared by UV-initiated photo-polymerization of HEMA and MAGA-Fe3+ complex in the presence of an initiator (benzoyl peroxide). After that, the template (i.e., Fe3+ ions) was removed by using 0.1 M EDTA solution at room temperature. The specific surface area of the Fe3+-imprinted poly(HEMA-MAGA) membranes was found to be 49.2 m(2)/g and the swelling ratio was 92%. According to the elemental analysis results, the polymeric membranes contained 145.7 mu mol MAGA/g polymer. The maximum adsorption capacity was 164.2 mu mol Fe3+/g membrane. The relative selectivity coefficients of ion-imprinted membranes for Fe3+/Zn2+ and Fe3+/Cr3+ were 12.6 and 62.5 times greater than the non-imprinted matrix, respectively. The Fe3+-imprinted poly( HEMA- MAGA) membranes could be used many times without decreasing their Fe3+ adsorption capacities significantly.