Preliminary characterization of Rhizobium strains isolated from chickpea nodules

KÜÇÜK Ç., KIVANÇ M.

AFRICAN JOURNAL OF BIOTECHNOLOGY, vol.7, no.6, pp.772-775, 2008 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 7 Issue: 6
  • Publication Date: 2008
  • Journal Name: AFRICAN JOURNAL OF BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.772-775
  • Keywords: Rhizobium, chickpea (Cicer arietinum L.), preliminary characterization, INTRINSIC ANTIBIOTIC-RESISTANCE, SYMBIOTIC NITROGEN-FIXATION, N-2 FIXATION, SALT, LEGUMES, GROWTH
  • Anadolu University Affiliated: Yes

Abstract

A total of 28 Rhizobium strains from chickpea nodules were characterized on the basis of morphological, cultural and biochemical characteristics. Most strains produced abundant extracellular polysaccharides, were tolerant to 0.5 M NaCl (53%) and a temperature of 40 degrees C (75%). The majority of the strains showed an intrinsic resistance to the antibiotics (mu g ml(-1)) streptomycin ( 100), kanamycin (50), erytromycin (30), chloramphenicol (200) and penicilin (25). In vitro antibiosis assays indicated that Rhizobium strains from chickpea nodules exercised an antagonism against Ascocyhta rabiei the agent of ascocyhta blight disease of chickpea.