© 2019, Badebio Biotechnololgy Ltd. All rights reserved.The volatiles obtained by hydrodistillation of Hirtellina lobelii (DC.) Dittrich (Asteraceae) collected from Turkey was analyzed by gas chromatography and gas chromatography/mass spectrometry (GC/MS), simultaneously. Main constituents were identified as fokienol (% 7.4), cadinol (% 6.8), and β-caryophyllene (% 6.7), respectively. The H. lobelii volatiles were screened for antimicrobial properties against various pathogenic bacteria. The volatile showed a relatively good inhibitory concentration with 0.06 mg/mL against Staphylococcus aureus.