Effects of thapsigargicin on Ca2+ movements in L1210 cells permeabilized with digitonin

ÖZTETİK E.

Biomeditsinskaya Khimiya, vol.55, no.5, pp.651-662, 2009 (ESCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 55 Issue: 5
  • Publication Date: 2009
  • Doi Number: 10.1134/s1990750809020061
  • Journal Name: Biomeditsinskaya Khimiya
  • Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus
  • Page Numbers: pp.651-662
  • Keywords: calcium, intracellular calcium, L1210 mouse lymphoma cells, thapsigargicin, thapsigargin
  • Anadolu University Affiliated: Yes

Abstract

The effect of Thapsigargicin (TGC), a non-phorbol ester type tumor promoter, on Ca2+ movements has been investigated using L1210 mouse lymphoma cells. Ca2+ release from intact and digitonin permeabilized cells was evaluated using Fura-2 and Fura-3. TGC like Thapsigargin (TG) has the ability to discharge the intracellular Ca2+ stores and to increase intracellular free Ca2+ concentrations. TGC in a concentration dependent manner (0.16-16 nM) also inhibited cell growth and this effect was at least partially reversed by arachidonate.