Chloroquine Used in Combination with Chemotherapy Synergistically Suppresses Growth and Angiogenesis In Vitro and In Vivo

Creative Commons License

Gurel-Gurevin E., KIYAN H. T., ESENER O. B. B., Aydinlik S., ÜVEZ A., ULUKAYA E., ...Daha Fazla

ANTICANCER RESEARCH, cilt.38, sa.7, ss.4011-4020, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 38 Sayı: 7
  • Basım Tarihi: 2018
  • Doi Numarası: 10.21873/anticanres.12689
  • Dergi Adı: ANTICANCER RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.4011-4020
  • Anahtar Kelimeler: Doxorubicin, chloroquine, angiogenesis, CAM assay, tube formation, CHORIOALLANTOIC MEMBRANE ASSAY, CANCER-THERAPY, TUMOR ANGIOGENESIS, BREAST-CANCER, CELL-LINE, DOXORUBICIN, MODEL, INHIBITION, METASTASIS, EFFICACY
  • Anadolu Üniversitesi Adresli: Evet

Özet

Background: The inhibition of autophagy using pharmacological inhibitors such as chloroquine may be an effective strategy to overcome chemotherapy or resistance to anti-angiogenic therapy. Materials and Methods: The cytotoxic effect of doxorubicin (0.1-1 mu M), chloroquine (0.25-32 ,mu M) and their combination were investigated by employing ATP assay in human umbilical vein endothelial cells (HUVECs). The effect of doxorubicin and chloroquine combination was also measured using tube formation assay on Matrigel. The anti-angiogenic activities of doxorubicin (2.5 mu g/pellet) and chloroquine (15 mu g/pellet), their combination, and standards (50 mu g/pellet) were tested in vivo using the chick embryo chorioallantoic membrane (CAM) assay. Results: The combination of doxorubicin and chloroquine significantly had a stronger anti-angiogenic effect than the positive control (+/-)-thalidomide and doxorubicin alone in the CAM assay and in vitro tube-formation assay. Conclusion: Chloroquine enhanced the anti-angiogenic effect of doxorubicin on CAM at the tested concentrations.