Preparation and characterization of ascorbic acid loaded solid lipid nanoparticles and investigation of their apoptotic effects

GÜNEY G., KUTLU H. M., Genc L.

COLLOIDS AND SURFACES B-BIOINTERFACES, cilt.121, ss.270-280, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 121
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1016/j.colsurfb.2014.05.008
  • Dergi Adı: COLLOIDS AND SURFACES B-BIOINTERFACES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.270-280
  • Anahtar Kelimeler: Solid lipid nanoparticles (SLNs), Ascorbic acid (AA), Cancer, Apoptotic effect, Cytotoxic activity, VITAMIN-C, DRUG-DELIVERY, CANCER-CELLS, PHARMACOKINETICS, CYTOTOXICITY, SLN
  • Anadolu Üniversitesi Adresli: Evet

Özet

In this paper, ascorbic acid (Vitamin C, AA) known as an antioxidant was successfully incorporated in solid lipid nanoparticles (SLNs) by hot homogenization and efficient delivery of AA to cancer cells. The obtained SLN formulations were characterized by Nano Zetasizer ZS and HPLC with the particle size being less than 250 nm. AA-SLNs exhibited sustained release and high entrapment efficiency. According to MTT test results, AA-SLNs showed high cytotoxic activity compared to the free AA against H-Ras 5RP7 cells without damaging NIH/3T3 control cells. These results were supported by the Annexin V-PI and caspase-3 assay. Furthermore, as compared to the M, AA-SLNs exhibited more efficient cellular uptake, accumulated in the cytoplasm and induced apoptosis which was observed by confocal laser scanning microscopy (CLSM) and transmission electron microscopy (TEM). Thus, the results of this study suggest that SLNs can be a potential nanocarrier system for AA. (C) 2014 Elsevier B.V. All rights reserved.