Radiolabeling and quality control studies of [99mTc]Tc-dexketoprofen trometamol for inflammation imaging

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Ekinci M., Öztürk A. A., İlem Özdemir D.

Experimental Biomedical Research, cilt.8, sa.1, ss.1-7, 2025 (Hakemli Dergi)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 8 Sayı: 1
  • Basım Tarihi: 2025
  • Doi Numarası: 10.30714/j-ebr.2025.231
  • Dergi Adı: Experimental Biomedical Research
  • Derginin Tarandığı İndeksler: TR DİZİN (ULAKBİM), Index Copernicus
  • Sayfa Sayıları: ss.1-7
  • Anadolu Üniversitesi Adresli: Evet

Özet

Aim: To radiolabel dexketoprofen trometamol (DT) with [99mTc]Tc for developing inflammation imaging agent.


Method: For this purpose, DT was radiolabeled using [99mTc]Tc, and radioactive thin layer chromatography (RTLC) was used in the quality control studies of [99mTc]Tc-DT. In addition, the effects of quality control factors (reducing agent, incubation time, pH value) on radiolabeling were studied. Finally, the stability study of [99mTc]Tc-DT was performed and the partition coefficient value of [99mTc]Tc-DT was calculated.


Results: Based on the results, a new, simple, and fast direct technique was used for producing [99mTc]Tc-DT with over 92% labeling efficiency after 15 minutes of pH 9.0 incubation. 37 MBq [99mTc]Tc, 10 µg of stannous chloride dihydrate (reducing agent), and 250 µg of DT were utilized to produce the ideal radiolabeling conditions. According to the RTLC experiments, [99mTc]Tc-DT remained stable at room temperature for six hours. The [99mTc]Tc-DT logP has been determined to be -0.48 ± 0.02.


Conclusions: Based on the acquired results, [99mTc]Tc-DT has the potential to be an imaging agent for inflammation. Further studies are in progress, such as biodistribution of [99mTc]Tc-DT.


Keywords: Dexketoprofen trometamol, radiolabeling, radiopharmaceuticals, technetium-99m.