Effects of some drugs on enzymatic activity of glucose 6-phosphate dehydrogenase from chicken erythrocytes in vitro

Ciftci M., BEYDEMİR Ş., Ekinci D.

Asian Journal of Chemistry, vol.20, no.3, pp.2189-2196, 2008 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 20 Issue: 3
  • Publication Date: 2008
  • Journal Name: Asian Journal of Chemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2189-2196
  • Keywords: Chicken, Drug, G6PD enzyme, Inhibition
  • Anadolu University Affiliated: Yes


In vitro inhibitory effects of some important drugs on glucose 6-phosphate dehydrogenase, the NADP+ depending enzyme, from the chicken erythrocytes were investigated in the peresent study. Chicken erythrocytes glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate: NADP+ oxidoreductase, EC (G6PD) was purified by using 2′,5′-ADP Sepharose 4B affinity chromatography. During the overall purification steps, the enzyme having a specific activity of 17.90 EU/mg proteins was obtained 5343-fold with a yield of 52 %. A constant temperature (4°C) was maintained during the purification process. Enzyme activity was determined with the Beutler's method by using a spectrophotometer at 340 nm. This method was utilized for all kinetic studies. Then, metamizol, ceftriaxone, prilocaine, meloksikam, lidocaine were investigated for in vitro inhibition of this NADP+ depending enzyme. The enzyme was strongly inhibited by these drugs. In addition, I50 values of the drugs were determined by plotting activity % vs. drug concentrations. I50 values were 0.57 mM for metamizol, 0.33 mM for ceftriaxone, 28.17 mM for prilocaine, 0.0012 mM for meloksikam and 1.54 mM for lidocaine.