Effects of some drugs on enzymatic activity of glucose 6-phosphate dehydrogenase from chicken erythrocytes in vitro
Asian Journal of Chemistry, cilt.20, sa.3, ss.2189-2196, 2008 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 20 Sayı: 3
- Basım Tarihi: 2008
- Dergi Adı: Asian Journal of Chemistry
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.2189-2196
- Anahtar Kelimeler: Chicken, Drug, G6PD enzyme, Inhibition
- Anadolu Üniversitesi Adresli: Evet
Özet
In vitro inhibitory effects of some important drugs on glucose 6-phosphate dehydrogenase, the NADP+ depending enzyme, from the chicken erythrocytes were investigated in the peresent study. Chicken erythrocytes glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate: NADP+ oxidoreductase, EC 1.1.1.49) (G6PD) was purified by using 2′,5′-ADP Sepharose 4B affinity chromatography. During the overall purification steps, the enzyme having a specific activity of 17.90 EU/mg proteins was obtained 5343-fold with a yield of 52 %. A constant temperature (4°C) was maintained during the purification process. Enzyme activity was determined with the Beutler's method by using a spectrophotometer at 340 nm. This method was utilized for all kinetic studies. Then, metamizol, ceftriaxone, prilocaine, meloksikam, lidocaine were investigated for in vitro inhibition of this NADP+ depending enzyme. The enzyme was strongly inhibited by these drugs. In addition, I50 values of the drugs were determined by plotting activity % vs. drug concentrations. I50 values were 0.57 mM for metamizol, 0.33 mM for ceftriaxone, 28.17 mM for prilocaine, 0.0012 mM for meloksikam and 1.54 mM for lidocaine.