Effect of α-tocopherol oil antioxidant enzyme activities and lipid peroxidation in rainbow trout (<i>Oncorhynchus mykiss</i>)

GÜLÇİN İ., BEYDEMİR Ş., Hisar O.

ACTA VETERINARIA HUNGARICA, no.4, pp.425-433, 2005 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Publication Date: 2005
  • Journal Name: ACTA VETERINARIA HUNGARICA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.425-433
  • Anadolu University Affiliated: No

Abstract

In this Study, the effect of alpha-tocopherol on the antioxidant capacity of rainbow trout (Oncorhynchus mykiss) was determined. For this purpose, activities of antioxidant enzymes Such as catalase (CAT), peroxidase (POD) and glutathione reductase (GSSG-Rx) were investigated. Enzyme activities were measured at 0 (control), 1, 3 and 5 h after alpha-tocopherol injection. In addition, the levels of malondialdehyde (MDA) as a lipid peroxidation marker were determined in the erythrocytes. The results showed that alpha-tocopherol significantly activated the CAT, POD and GSSG-Rx enzymes as compared with the enzyme activities found in the controls (p < 0.05). However, MDA levels were significantly decreased by alpha-tocopherol treatment (p < 0.05). The results Suggest that alpha-tocopherol may have a pro-oxidant tendency at a high dose and cause mild oxidative stress which Could modulate signal transduction cascades, redirect gene expression, and influence Many Cellular responses such as proliferation, differentiation, and reproduction. For this reason, a-tocopherol should be used carefully in all applications in relation to fish.