Studies on the cytotoxic, apoptotic and antitumoral effects of Au(III) and Pt(II) complexes of 1, 10-phenanthroline on V79 379A and A549 cell lines

Bostancioglu R. B., Isik K., Genc H., BENKLİ K., Koparal A. T.

JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, cilt.27, sa.3, ss.458-466, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 27 Sayı: 3
  • Basım Tarihi: 2012
  • Doi Numarası: 10.3109/14756366.2011.596835
  • Dergi Adı: JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.458-466
  • Anahtar Kelimeler: Au(III), Pt(II), cytotoxicity, apoptosis, MTT assay, DNA fragmentation, colony forming ability assay (CFA), DAPI staining, active caspase-3, PLATINUM(II) COMPLEXES, GOLD(III) COMPLEXES, SOLUTION CHEMISTRY, METAL-COMPLEXES, IN-VITRO, ANTICANCER DRUGS, CANCER-CELLS, BINDING, 1,10-PHENANTHROLINE, DERIVATIVES
  • Anadolu Üniversitesi Adresli: Evet

Özet

In the present study, Au(III) and Pt(II) complexes of 1, 10-phenanthroline (phen) were synthesized and used as the test compounds. The structure elucidation of the synthesized compounds was performed by IR, H-1-NMR and MASS spectroscopic data and the results of elemental analyses. The cytotoxic and apoptotic effects of test compounds were elucidated on V79 379A (Chinese hamster lung fibroblast like) and A549 (human lung carcinoma epithelial like) cell lines. Cytotoxicity was measured with MTT assay and antitumoral effect was determined by colony forming ability methods. In addition, nuclear fragmentation and activation of apoptotic enzyme (caspase-3) and DAPI staining were used to detect the apoptotic effect of the compounds. All the test compounds induced time and concentration-dependent cytotoxic and antitumoral effects. Significant increases in the levels of apoptosis were observed with increasing exposure concentration.