Flow injection analysis of mitomycin-C in flacons

Dal A. G., Dogrukol-Ak D., TUNÇEL M.

Journal of Liquid Chromatography and Related Technologies, vol.28, no.4, pp.619-629, 2005 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 28 Issue: 4
  • Publication Date: 2005
  • Doi Number: 10.1081/jlc-200047224
  • Journal Name: Journal of Liquid Chromatography and Related Technologies
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.619-629
  • Keywords: mitomycin-C, flow injection analysis, pharmaceutical analysis, validation, PERFORMANCE LIQUID-CHROMATOGRAPHY, SPECTROPHOTOMETRIC DETERMINATION, ANTICANCER DRUGS, ANALYSIS FIA, UV-DETECTION, URINE, PLASMA, ASSAY, SERUM, TISSUE
  • Anadolu University Affiliated: Yes

Abstract

A precise and accurate flow injection analysis (FIA) method for the determination of mitomycin-C (MMC) in pharmaceutical flacons is described. Britton-Robinson (BR) buffer at pH 7.0 was used as a carrier solvent at a flow-rate of 1.0 mL min-1. MMC signals were detected at 363 nm using a UV-spectrophotometric detector. The intra- and inter-assay precisions were less than 2.0%. The calibration curves were also linear in the concentration range of 2.0 × 10-6-1.0 × 10-5 M for intra- and inter-day studies with high correlation coefficients. The limit of detection (LOD) and limit of quantification (LOQ) values were found to be 1.93 × 10-8 and 6.45 × 10-8 M, respectively. The results obtained with the application of the method were compared with those of high performance liquid chromatography (HPLC) and UV-spectrophotometry. It was found that there was insignificant difference between the methods.