DNA ligase photocrosslinked cryogenic column based biotinylation kit for viral hybridization and detection

Kose S. O., ÖZİÇ C., YILMAZ F., ERSÖZ A., Say R.

PROCESS BIOCHEMISTRY, cilt.84, ss.213-219, 2019 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 84
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1016/j.procbio.2019.06.011
  • Dergi Adı: PROCESS BIOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.213-219
  • Anahtar Kelimeler: Crimean Congo hemorrhagic fever (CCHF), DNA ligase, Cryogel columns, Quantum dots (QDs), Biotinylation, Unneeded ATP biotinylation process, HEMORRHAGIC-FEVER VIRUS, POLYMERASE-CHAIN-REACTION, QUANTUM DOTS, NUCLEIC-ACID, IXODID TICKS, LIGATION, FRET
  • Anadolu Üniversitesi Adresli: Evet

Özet

This study describes the development of cryogel columns prepared by ATP having ruthenium hapten cross-linker based photosensitive approach "ANADOLUCA" for the biotinylation of Crimean Congo Hemorrhagic Fever (CCHF) primers through 5' end as a first. In this process, which does not require ATP ligand, CCHF recognition has been performed using stable and quantum dots (QDs) based avidity nanoparticles cross-linked with streptavidin.