PROCESS BIOCHEMISTRY, vol.84, pp.213-219, 2019 (SCI-Expanded)
Article / Article
Science Citation Index Expanded (SCI-EXPANDED), Scopus
Crimean Congo hemorrhagic fever (CCHF), DNA ligase, Cryogel columns, Quantum dots (QDs), Biotinylation, Unneeded ATP biotinylation process, HEMORRHAGIC-FEVER VIRUS, POLYMERASE-CHAIN-REACTION, QUANTUM DOTS, NUCLEIC-ACID, IXODID TICKS, LIGATION, FRET
Anadolu University Affiliated:
This study describes the development of cryogel columns prepared by ATP having ruthenium hapten cross-linker based photosensitive approach "ANADOLUCA" for the biotinylation of Crimean Congo Hemorrhagic Fever (CCHF) primers through 5' end as a first. In this process, which does not require ATP ligand, CCHF recognition has been performed using stable and quantum dots (QDs) based avidity nanoparticles cross-linked with streptavidin.