DNA ligase photocrosslinked cryogenic column based biotinylation kit for viral hybridization and detection

Kose S. O., ÖZİÇ C., YILMAZ F., ERSÖZ A., Say R.

PROCESS BIOCHEMISTRY, vol.84, pp.213-219, 2019 (SCI-Expanded, Scopus)

  • Publication Type: Article / Article
  • Volume: 84
  • Publication Date: 2019
  • Doi Number: 10.1016/j.procbio.2019.06.011
  • Journal Name: PROCESS BIOCHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.213-219
  • Keywords: Crimean Congo hemorrhagic fever (CCHF), DNA ligase, Cryogel columns, Quantum dots (QDs), Biotinylation, Unneeded ATP biotinylation process, HEMORRHAGIC-FEVER VIRUS, POLYMERASE-CHAIN-REACTION, QUANTUM DOTS, NUCLEIC-ACID, IXODID TICKS, LIGATION, FRET
  • Anadolu University Affiliated: Yes

Abstract

This study describes the development of cryogel columns prepared by ATP having ruthenium hapten cross-linker based photosensitive approach "ANADOLUCA" for the biotinylation of Crimean Congo Hemorrhagic Fever (CCHF) primers through 5' end as a first. In this process, which does not require ATP ligand, CCHF recognition has been performed using stable and quantum dots (QDs) based avidity nanoparticles cross-linked with streptavidin.