In vitro cytotoxic activities of (+)-usnic acid and (-)-usnic acid on V79, A549, and human lymphocyte cells and their non-genotoxicity on human lymphocytes

Koparal A. T., Tueylue B. A., Tuerk H.

NATURAL PRODUCT RESEARCH, cilt.20, sa.14, ss.1300-1307, 2006 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 20 Sayı: 14
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1080/14786410601101910
  • Dergi Adı: NATURAL PRODUCT RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1300-1307
  • Anahtar Kelimeler: usnic acid, cytotoxicity, genotoxicity, Ramalina farinacea, Cladonia foliacea, USNIC ACID, ANTIMICROBIAL ACTIVITY, LICHEN, CONSTITUENTS, APOPTOSIS, EXTRACTS, STRESS
  • Anadolu Üniversitesi Adresli: Evet

Özet

This study investigates cytotoxic and genotoxic activities of (+)-Usnic acid and (-)-usnic acid isolated from the lichen Ramalina farinacea and the lichen Cladonia foliacea, respectively. To determine the activities of these acids, we used the MTT assay on V79 (Chinese hamster lung fibroblast like) and A549 (human lung carcinoma epithelial like) cell lines and cytokinesis-blocked micronucleus (CBMN) assay in human lymphocytes in vitro. Our results suggest that both enantiomers of usnic acid are non-genotoxic shown by the absence of micronucleus induction in human lymphocytes and have significant cytotoxic and apoptotic effects to induce cell killing in cultured human lymphocytes, V79 and A549 cell lines. Even low doses of (+)-usnic acid showed high cytotoxic activity against cancerous cells. The MTT results and cell proliferation index (CPI) values based on the CBMN test results are found in good agreement.