Preparation of poly(3,4-ethylenedioxythiophene) nanofibers modified pencil graphite electrode and investigation of over-oxidation conditions for the selective and sensitive determination of uric acid in body fluids

ÖZCAN A., Ilkbaş S.

Analytica Chimica Acta, cilt.891, ss.312-320, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 891
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1016/j.aca.2015.08.015
  • Dergi Adı: Analytica Chimica Acta
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.312-320
  • Anahtar Kelimeler: Uric acid, Ascorbic acid, Poly(3,4-ethylenedioxythiophene), Over-oxidation, Blood serum, Urine, ELECTROCHEMICAL SIMULTANEOUS DETERMINATION, ASCORBIC-ACID, GRAPHENE OXIDE, DOPED POLY(3,4-ETHYLENEDIOXYTHIOPHENE), ELECTROCATALYTIC OXIDATION, SENSING PLATFORM, CARBON NANOTUBES, DOPAMINE, POLYPYRROLE, PEDOT
  • Anadolu Üniversitesi Adresli: Evet

Özet

© 2015 Elsevier B.V.In this study, we have performed the preparation of over-oxidized poly(3,4-ethylenedioxythiophene) nanofibers modified pencil graphite electrode (Ox-PEDOT-nf/PGE) to develop a selective and sensitive voltammetric uric acid (UA) sensor. It was noted that the over-oxidation potential and time had a prominent effect on the UA response of the Ox-PEDOT-nf/PGE. Characterizations of PEDOT-nf/PGE and Ox-PEDOT-nf/PGE have been performed by cyclic voltammetry, electrochemical impedance spectroscopy, scanning electron microscopy, Fourier transform infrared spectroscopy and Raman spectroscopy. The highest voltammetric response of UA was obtained at pH 2.0. A linear relationship between the concentration of UA and oxidation peak currents was observed in the concentration range of 0.01-20.0 μM. The detection limit (1.3 nM according to S/N = 3) and reproducibility (RSD: 4.6 % for N:10) have also been determined. The effects of different substances on the determination of UA have been investigated. A very high peak separation value of 423 mV was obtained between UA and ascorbic acid which is the major interfering substance for UA. The use of Ox-PEDOT-nf/PGE has been successfully tested in the determination of UA in human blood serum and urine samples for the first time in the literature.