The in Vitro Effects of Dexamethasone on Sheep Lens Glucose-6-Phosphate Dehydrogenase

BEYDEMİR Ş., Kulaçoǧlu D. N., Çiftçi M., KÜFREVİOĞLU Ö. İ.

Turkish Journal of Chemistry, vol.27, no.5, pp.601-608, 2003 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 27 Issue: 5
  • Publication Date: 2003
  • Journal Name: Turkish Journal of Chemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.601-608
  • Keywords: Dexamethasone, Glucose-6-phosphate dehydrogenase, Sheep eye lens
  • Anadolu University Affiliated: Yes

Abstract

Glucose-6-phosphate dehydrogenase (G6PD) enzyme was purified from sheep lenses, and the in vitro effects of dexamethasone on the enzyme's activity were investigated. Sheep lens glucose-6-phosphate dehydrogenase was purified 10,000-fold by using ammonium sulphate fractionation and 2′,5′-ADP- Sepharose 4B affinity gel chromatography for a yield of 83.8% and a specific activity of 7.6 EU/mg protein. Enzyme activity was determined by Beutler's method. Dexamethasone strongly inhibited the enzyme under in vitro conditions. The I50 value of the dexamethasone was 3.05 mM.