Development and validation of a new HPLC method for the determination of quetiapine and its metabolites 7-hydroxy quetiapine and quetiapine sulfoxide in rat plasma Sıçan plazmasında ketiapin ve metabolitleri 7-hidroksi ketiapin ve ketiapin sülfoksit’in tayini için yeni bir yüksek performanslı sıvı kromatografisi yöntemi geliştirilmesi ve yöntemin validasyonu

ATİLA KARACA S., Yilmaz I. T., ŞENER E., YENİCELİ UĞUR D.

Turkish Journal of Pharmaceutical Sciences, cilt.13, sa.1, ss.17-26, 2016 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 13 Sayı: 1
  • Basım Tarihi: 2016
  • Dergi Adı: Turkish Journal of Pharmaceutical Sciences
  • Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.17-26
  • Anahtar Kelimeler: 7-Hydroxy quetiapine, HPLC, Quetiapine, Quetiapine sulfoxide, Rat plasma
  • Anadolu Üniversitesi Adresli: Evet

Özet

© 2016, Turkish Pharmacists Association. All rights reserved.A new high performance liquid chromatography method was developed and validated for the determination of quetiapine and its metabolites 7-hydroxy quetiapine and quetiapine sulfoxide in rat plasma. Separation was performed on a C18 column (Zorbax Eclipse Plus 4.6 mm ×100 mm, 3.5 μm particles) using a gradient elution at a flow rate of 1 mL/min. Mobile phase consisted of acetate buffer (10 mM, pH5) and acetonitrile. Analytes were detected with a DAD detector at 225 nm. Carbamazepine was used as internal standard in all analyses. Plasma samples were analyzed after a simple, one-step protein precipitation with acetonitrile. Separation time was 15 min including clean-up step. The method was validated in terms of precision, accuracy, recoveries, matrix effect and stability. It was found to be linearin the range of 0.065-130 μg/mL for quetiapine, 0.086-171 μg/mL for 7-hydroxy quetiapine and 0.042-83.35 μg/mL for quetiapine sulfoxide. All validation parameters were acceptable. This method was successfully applied to quantify the concentrations of the analytes in rat plasma.