Optimization of Alkaline Pretreatment for Enzymatic Saccharification of Poppy Stalks

Uzunlu N., HOŞGÜN E. Z., BOZAN B.

BIORESOURCES, vol.9, no.2, pp.2824-2834, 2014 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 9 Issue: 2
  • Publication Date: 2014
  • Doi Number: 10.15376/biores.9.2.2824-2834
  • Journal Name: BIORESOURCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2824-2834
  • Keywords: Poppy stalks, Alkaline pretreatment, Enzymatic saccharification, Optimization, LIGNOCELLULOSIC BIOMASS, EXTRUSION PRETREATMENT, ETHANOL-PRODUCTION, SODIUM-HYDROXIDE, STRAW, HYDROLYSIS, HEMICELLULOSES, TECHNOLOGIES, PEROXIDE, POPLAR
  • Anadolu University Affiliated: Yes

Abstract

Response surface methodology (RSM) was employed to optimize dilute alkaline pretreatment conditions for the maximum glucose yield of poppy stalk, with respect to NaOH concentration (1.0 to 3.5%, w/v), pretreatment temperature (50 to 100 degrees C), and pretreatment time (10 to 110 min). Recovery of glucan ranged from 61.02% to 99.14%, based on the initial glucan in the raw material. The highest lignin removal (43.43%) was obtained at the pretreatment conditions of 90 degrees C and 3.50% NaOH for 90 min. The optimum pretreatment conditions for maximum glucose yield after enzymatic hydrolysis were found to be 2.40% w/v NaOH, 70 min, and 80 degrees C. Under these conditions, experimental glucose and xylose yields were 499.35 mg/g glucan and 498.66 mg xylose/g xylan, respectively.