Effects of Thapsigargicin on Ca2+ Movements in L1210 Cells Permeabilized with Digitonin
BIOCHEMISTRY MOSCOW-SUPPLEMENT SERIES B-BIOMEDICAL CHEMISTRY, cilt.3, sa.2, ss.156-163, 2009 (ESCI, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 3 Sayı: 2
- Basım Tarihi: 2009
- Doi Numarası: 10.1134/s1990750809020061
- Dergi Adı: BIOCHEMISTRY MOSCOW-SUPPLEMENT SERIES B-BIOMEDICAL CHEMISTRY
- Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus
- Sayfa Sayıları: ss.156-163
- Anahtar Kelimeler: calcium, intracellular calcium, L1210 mouse lymphoma cells, thapsigargicin, thapsigargin
- Anadolu Üniversitesi Adresli: Evet
Özet
The effect of thapsigargicin (TGC), a non-phorbol ester type tumor promoter, on Ca2+ movements has been investigated using L1210 mouse lymphoma cells. Ca2+ release from intact and digitonin permeabilized cells was evaluated using Fura-2 and Fura-3. TGC like Thapsigargin (TG) has the ability to discharge the intracellular Ca2+ stores and to increase intracellular free Ca2+ concentrations. TGC in a concentration dependent manner (0.16-16 nM) also inhibited cell growth and this effect was at least partially reversed by arachidonate.