Effects of Thapsigargicin on Ca2+ Movements in L1210 Cells Permeabilized with Digitonin

Oztetik E.

BIOCHEMISTRY MOSCOW-SUPPLEMENT SERIES B-BIOMEDICAL CHEMISTRY, cilt.3, sa.2, ss.156-163, 2009 (ESCI) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 3 Sayı: 2
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1134/s1990750809020061
  • Dergi Adı: BIOCHEMISTRY MOSCOW-SUPPLEMENT SERIES B-BIOMEDICAL CHEMISTRY
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus
  • Sayfa Sayıları: ss.156-163
  • Anahtar Kelimeler: calcium, intracellular calcium, L1210 mouse lymphoma cells, thapsigargicin, thapsigargin
  • Anadolu Üniversitesi Adresli: Evet

Özet

The effect of thapsigargicin (TGC), a non-phorbol ester type tumor promoter, on Ca2+ movements has been investigated using L1210 mouse lymphoma cells. Ca2+ release from intact and digitonin permeabilized cells was evaluated using Fura-2 and Fura-3. TGC like Thapsigargin (TG) has the ability to discharge the intracellular Ca2+ stores and to increase intracellular free Ca2+ concentrations. TGC in a concentration dependent manner (0.16-16 nM) also inhibited cell growth and this effect was at least partially reversed by arachidonate.