Akademik Veri Yönetim Sistemi
International Nuclear Science and Technologies (INSTEC-2024), İzmir, Türkiye, 5 - 07 Eylül 2024, ss.67
In this study, dexketoprofen trometamol (DT) loaded chitosan-based nanoparticles were
radiolabeled with technetium-99m ([99mTc]Tc), and quality control and in vitro stability studies
of the radiolabeled nanoparticles were performed. First, DT loaded chitosan-based
nanoparticles were prepared spray drying technique. According to obtained results,
nanoparticles with zeta potential of +48.7±2.1 mV, particle size of 650.8±10.2 nm,
polydispersity index of 0.437±0.052, and encapsulation efficiency of 75.1±1.5% were
successfully developed. Then, nanoparticles were radiolabeled with [99mTc]Tc using the
stannous tartrate method, and quality control experiments for [99mTc]Tc-DT-chitosan
nanoparticles were performed using radioactive thin-layer chromatography (RTLC). In
addition, stability studies of [99mTc]Tc-DT-chitosan nanoparticles in different mediums (0.9%
sodium chloride solution, serum) were performed. According to the obtained results, [99mTc]Tc-
DT-chitosan nanoparticles were prepared with over 95% labeling efficiency by a novel, easy,
and quick direct method with a 15 min incubation time at pH 9.0. To achieve the best
radiolabeling conditions, 1 mL nanoparticle formulation, 1 mg of reducing agent (stannous
tartrate), 0.1 mg of antioxidant agent (ascorbic acid), and 37 MBq [99mTc]Tc were used. Stability
studies indicated that [99mTc]Tc-DT-chitosan nanoparticles were stable for up to 6 h in 0.9%
sodium chloride solution at room temperature, and serum at 37°C. Further studies on the
biodistribution of radiolabeled nanoparticles in experimental animals for inflammation imaging
are in progress.