Many of the cave-dwelling bat species are under threat, because of destruction of caves such as filling or converting for other uses, human disturbance and loss of foraging habitats. In this study, cave-dwelling bat species and habitat preferences were investigated in Northwest of Central Anatolia in spring, summer and winter. Investigations were performed in 26 caves hosting (15) and not hosting (11) bats. Temperature, humidity, length of caves, distance to nearest settlement, paved road, water source, agricultural area; height, width and orientation of cave entrance and activity of cave values were determined. Seven out of 10 recorded bat species were evaluated as new records for the area. There was a significant preference for caves which were situated at lower altitude, far from paved road and close to water source. Bats were mostly found in longer caves. Caves with entrance oriented to northwest and southeast were preferred by bats. Undertaking effective conservation measures to maintain especially eight threatened bat species which were found in the region should be a priority for conservation management plans.