Screening of free radical scavenging compounds in water extracts of Mentha samples using a postcolumn derivatization method

Kosar M., Dorman H., Baser K., Hiltunen R.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, cilt.52, sa.16, ss.5004-5010, 2004 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 52 Sayı: 16
  • Basım Tarihi: 2004
  • Doi Numarası: 10.1021/jf0496189
  • Dergi Adı: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.5004-5010
  • Anahtar Kelimeler: Mentha, 1,1-diphenyl-2-picrylhydrazyl (DPPH center dot), high-performance liquid chromatography, HPLC-DPPH center dot, antioxidant activity, free radicals, phenolics, ONLINE DETECTION, ANTIOXIDANT PROPERTIES, ESSENTIAL OILS, L., LAMIACEAE, HERBS
  • Anadolu Üniversitesi Adresli: Hayır

Özet

An on-line high-performance liquid chromatography-1,1-diphenyl-2-picrylhydrazyl (HPLC-DPPH.,) method has been improved for the detection of polar and nonpolar radical scavenging compounds in complex plant extracts. Nine water extracts were prepared from different Mentha species, varieties, hybrids, and cultivars. After the components within each extract had been separated by reverse phase chromatography using 10-100% methanol with 2% acetic acid as a mobile phase, analytes within the eluent capable of scavenging a citric acid-sodium citrate-buffered methanol 1,1-diphenyl-2-picrylhydrazyl solution were detected by postcolumn derivatization at 517 nm. The HPLC-DPPH. on-line method was applied to the qualitative and quantitative analysis of Mentha extracts. There was a strong correlation between the scavenging (negative) peak area and the concentration of the radical scavenging reference substances used. The minimum detectable concentration (mug/mL) of the antioxidant compounds was determined. Caffeic acid, eriocitrin (eriodictyol-7-O-rutinoside), luteolin7-O-glucoside, and rosmarinic acid were identified as the dominant radical scavengers in these extracts by this method.