Green, Rapid, Robust Determination of Aspartame in Beverages by High-Performance Liquid Chromatography-Evaporative Light Scattering Detection (HPLC-ELSD): Method Development and Validation

Soyseven M., Sezgin B., Acar E., Arli G.

ANALYTICAL LETTERS, no.11, pp.1773-1783, 2023 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Publication Date: 2023
  • Doi Number: 10.1080/00032719.2022.2146129
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Food Science & Technology Abstracts, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Page Numbers: pp.1773-1783
  • Keywords: Aspartame, beverages, green chemistry, high-performance liquid chromatography-evaporative light scattering detection (HPLC-ELSD), SOLID-PHASE EXTRACTION, ARTIFICIAL SWEETENERS, ACESULFAME, CYCLAMATE, CHEMISTRY, SUCRALOSE, PRODUCTS, FOODS
  • Anadolu University Affiliated: Yes


In recent years, eco-friendly approaches and reduction in the use of hazardous solvents have become important in applications of routine high-performance liquid chromatography (HPLC). Despite its various health side effects, aspartame (ASP) is a widely used artificial sweetener due to its high sweetness and low cost. In this study, a green, eco-friendly, cost effective, rapid, robust, and simple high-performance liquid chromatography-evaporative light scattering detection (HPLC-ELSD) method has been developed and validated for the determination of ASP in beverages. Separation was conducted isocratically on a C18 (4.6 x 150 mm, 3.5 mu m) column; the mobile phase consisted of 80:20 water:ethanol at a flow rate of 1 mL min(-1). The drift tube of evaporative light scattering detection (ELSD) temperature was adjusted to 50 degrees C and the nitrogen pressure was adjusted to 350 kPa. The HPLC-ELSD method provided a limit of detection and a limit of quantification of 0.33-0.99 mu g mL(-1), with recoveries of 99.4 +/- 0.68% and 102.8 +/- 0.17%, respectively. The precision of the method as the relative standard deviation was less than 1%. The method permitted the determination of ASP within 8 min using only 6.4 mL water and 1.6 mL ethanol. This study shows that the developed HPLC-ELSD method is suitable for determination of ASP in the food industry.