Inhibition of Human Neutrophil Responses by the Essential Oil of Artemisia kotuchovii and Its Constituents

Creative Commons License

Schepetkin I. A., Kushnarenko S. V., ÖZEK G., Kirpotina L. N., Utegenova G. A., Kotukhov Y. A., ...More

Journal of Agricultural and Food Chemistry, vol.63, no.20, pp.4999-5007, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 63 Issue: 20
  • Publication Date: 2015
  • Doi Number: 10.1021/acs.jafc.5b01307
  • Journal Name: Journal of Agricultural and Food Chemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.4999-5007
  • Keywords: Artemisia kotuchovii, calcium flux, chemotaxis, essential oil, neutrophil, N-formyl peptide receptor 1 (FPR1), reactive oxygen species, NF-KAPPA-B, L. ESSENTIAL OIL, CHEMICAL-COMPOSITION, VOLATILE COMPOUNDS, INFLAMMATION, CAROTENOIDS, DEGRADATION, SUPPRESSION, RECEPTORS, ELASTASE
  • Anadolu University Affiliated: Yes


© 2015 American Chemical Society.Essential oils were obtained by hydrodistillation of the flowers+leaves and stems of Artemisia kotuchovii Kupr. (AKEOf+l and AKEOstm, respectively) and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The primary components of the oils were estragole, (E)- and (Z)-β-ocimenes, methyleugenol, limonene, spathulenol, β-pinene, myrcene, and (E)-methyl cinnamate. Seventy-four constituents were present at concentrations from 0.1 to 1.0%, and 34 compounds were identified in trace (<0.1%) amounts in one or both plant components. Screening of the essential oils for biological activity showed that AKEOstm, but not AKEOf+l, inhibited N-formyl-Met-Leu-Phe (fMLF)-stimulated Ca2+ flux and chemotaxis and phorbol-12-myristate-13-acetate (PMA)-induced reactive oxygen species (ROS) production in human neutrophils. Selected pure constituents, representing >96% of the AKEOstm composition, were also tested in human neutrophils and HL-60 cells transfected with N-formyl peptide receptor 1 (FPR1). One component, 6-methyl-3,5-heptadien-2-one (MHDO), inhibited fMLF- and interleukin 8 (IL-8)-stimulated Ca2+ flux, fMLF-induced chemotaxis, and PMA-induced ROS production in human neutrophils. MHDO also inhibited fMLF-induced Ca2+ flux in FPR1-HL60 cells. These results suggest that MHDO may be effective in modulating some innate immune responses, possibly by inhibition of neutrophil migration and ROS production.