Inhibition of Human Neutrophil Responses by the Essential Oil of Artemisia kotuchovii and Its Constituents

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Schepetkin I. A., Kushnarenko S. V., ÖZEK G., Kirpotina L. N., Utegenova G. A., Kotukhov Y. A., ...Daha Fazla

Journal of Agricultural and Food Chemistry, cilt.63, sa.20, ss.4999-5007, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 63 Sayı: 20
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1021/acs.jafc.5b01307
  • Dergi Adı: Journal of Agricultural and Food Chemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.4999-5007
  • Anahtar Kelimeler: Artemisia kotuchovii, calcium flux, chemotaxis, essential oil, neutrophil, N-formyl peptide receptor 1 (FPR1), reactive oxygen species, NF-KAPPA-B, L. ESSENTIAL OIL, CHEMICAL-COMPOSITION, VOLATILE COMPOUNDS, INFLAMMATION, CAROTENOIDS, DEGRADATION, SUPPRESSION, RECEPTORS, ELASTASE
  • Anadolu Üniversitesi Adresli: Evet

Özet

© 2015 American Chemical Society.Essential oils were obtained by hydrodistillation of the flowers+leaves and stems of Artemisia kotuchovii Kupr. (AKEOf+l and AKEOstm, respectively) and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The primary components of the oils were estragole, (E)- and (Z)-β-ocimenes, methyleugenol, limonene, spathulenol, β-pinene, myrcene, and (E)-methyl cinnamate. Seventy-four constituents were present at concentrations from 0.1 to 1.0%, and 34 compounds were identified in trace (<0.1%) amounts in one or both plant components. Screening of the essential oils for biological activity showed that AKEOstm, but not AKEOf+l, inhibited N-formyl-Met-Leu-Phe (fMLF)-stimulated Ca2+ flux and chemotaxis and phorbol-12-myristate-13-acetate (PMA)-induced reactive oxygen species (ROS) production in human neutrophils. Selected pure constituents, representing >96% of the AKEOstm composition, were also tested in human neutrophils and HL-60 cells transfected with N-formyl peptide receptor 1 (FPR1). One component, 6-methyl-3,5-heptadien-2-one (MHDO), inhibited fMLF- and interleukin 8 (IL-8)-stimulated Ca2+ flux, fMLF-induced chemotaxis, and PMA-induced ROS production in human neutrophils. MHDO also inhibited fMLF-induced Ca2+ flux in FPR1-HL60 cells. These results suggest that MHDO may be effective in modulating some innate immune responses, possibly by inhibition of neutrophil migration and ROS production.