A novel, rapid and robust HPLC-ELSD method for simultaneous determination of fructose, glucose and sucrose in various food samples: Method development and validation

SOYSEVEN M., SEZGİN B., Arli G.

JOURNAL OF FOOD COMPOSITION AND ANALYSIS, vol.107, 2022 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 107
  • Publication Date: 2022
  • Doi Number: 10.1016/j.jfca.2022.104400
  • Journal Name: JOURNAL OF FOOD COMPOSITION AND ANALYSIS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Analytical Abstracts, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Food Science & Technology Abstracts, Veterinary Science Database
  • Keywords: HPLC, ELSD, Fructose, Glucose, Sucrose, Sugars, SUGAR-SWEETENED BEVERAGES, PERFORMANCE LIQUID-CHROMATOGRAPHY, SIMULTANEOUS SEPARATION, WEIGHT-GAIN, CHILDREN, SORBITOL, CONSUMPTION, PRODUCTS, XYLITOL, MALTOSE
  • Anadolu University Affiliated: Yes

Abstract

The analysis of sugar compounds in food products requires rapid, accurate and cost-effective methods. For this purpose, a novel and robust high-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) method was developed and validated for simultaneous determination of fructose, glucose and sucrose. Chromatographic separations were carried out on a Phenomenex Luna NH2 (5 mu m particle size, 250 mm x 4.6 mm id, 100 angstrom) column with isocratic elution with acetonitrile:water (78:22, v/v). Drift tube temperature was set at 40 degrees C and nitrogen pressure was regulated to 350 kPa with Gain:7 and Filter:10 on the ELSD system. Within the test ranges for all sugar compounds (40-2000 mu g mL(-1)), the regression equation revealed a good linear relationship (R-2 = 0.9980-0.9992) with low LOD and LOQ values in the range of 0.10-0.16 and 0.30-0.49 mu g mL(-1). The method was successfully applied to 36 food samples including honey, molasses, jams and fruits.