Characterization of volatile constituents of Haplopappus greenei and studies on the antifungal activity against phytopathogens

Demirci B., Baser K., Tabanca N., Wedge D.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol.54, no.8, pp.3146-3150, 2006 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 54 Issue: 8
  • Publication Date: 2006
  • Doi Number: 10.1021/jf053222v
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.3146-3150
  • Keywords: Haplopappus greenei, Asteraceae, essential oil, GC-MS, carvacrol, beta-pinene, strawberry pathogenic fungi, biological activity, antifungal activity, COMPARATIVE CHEMICAL-COMPOSITION, RESINOUS EXUDATE, CLERODANE DITERPENES, FOLIOSUS, FLAVONOIDS, UNCINATUS, ANTIBACTERIAL, MULTIFOLIUS, DESERTICOLA, COUMARINS
  • Anadolu University Affiliated: Yes


Essential oil of Haplopappus greenei A. Gray was obtained by hydrodistillation of aerial parts, which were subsequently analyzed by gas chromatography and gas chromatography-mass spectrometry. Major components were identified as carvacrol (8.7%), beta-pinene (7.6%), trans-pinocarveol (6.2%), and caryophyllene oxide (5.8%), respectively. In total, 104 components representing 84.9% of the investigated essential oil were characterized. Furthermore, the essential oil was evaluated for antimalarial, antimicrobial, and antifungal activities. However, only antifungal activity was observed against the strawberry anthracnose-causing fungal plant pathogens Colletotrichum acutatum, Colletotrichum fragariae, and Colletotrichum gloeosporioides using the direct overlay bioautography assay. Major essential oil components were also evaluated for antifungal activity; the carvacrol standard demonstrated nonselective activity against the three Colletotrichum species and the other compounds were inactive.