Characterization of volatile constituents of Haplopappus greenei and studies on the antifungal activity against phytopathogens
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol.54, no.8, pp.3146-3150, 2006 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 54 Issue: 8
- Publication Date: 2006
- Doi Number: 10.1021/jf053222v
- Journal Name: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.3146-3150
- Keywords: Haplopappus greenei, Asteraceae, essential oil, GC-MS, carvacrol, beta-pinene, strawberry pathogenic fungi, biological activity, antifungal activity, COMPARATIVE CHEMICAL-COMPOSITION, RESINOUS EXUDATE, CLERODANE DITERPENES, FOLIOSUS, FLAVONOIDS, UNCINATUS, ANTIBACTERIAL, MULTIFOLIUS, DESERTICOLA, COUMARINS
- Anadolu University Affiliated: Yes
Abstract
Essential oil of Haplopappus greenei A. Gray was obtained by hydrodistillation of aerial parts, which were subsequently analyzed by gas chromatography and gas chromatography-mass spectrometry. Major components were identified as carvacrol (8.7%), beta-pinene (7.6%), trans-pinocarveol (6.2%), and caryophyllene oxide (5.8%), respectively. In total, 104 components representing 84.9% of the investigated essential oil were characterized. Furthermore, the essential oil was evaluated for antimalarial, antimicrobial, and antifungal activities. However, only antifungal activity was observed against the strawberry anthracnose-causing fungal plant pathogens Colletotrichum acutatum, Colletotrichum fragariae, and Colletotrichum gloeosporioides using the direct overlay bioautography assay. Major essential oil components were also evaluated for antifungal activity; the carvacrol standard demonstrated nonselective activity against the three Colletotrichum species and the other compounds were inactive.