Comparison of the toxicity of pure compounds and commercial formulations of imidacloprid and acetamiprid on HT-29 cells: Single and mixture exposure

BAYSAL M., ATLI EKLİOĞLU Ö.

FOOD AND CHEMICAL TOXICOLOGY, cilt.155, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 155
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.fct.2021.112430
  • Dergi Adı: FOOD AND CHEMICAL TOXICOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, EMBASE, Environment Index, Food Science & Technology Abstracts, MEDLINE, Pollution Abstracts, Veterinary Science Database
  • Anahtar Kelimeler: Neonicotinoids, HT-29 cell line, Apoptosis, Oxidative stress, DNA damage, Mixture toxicity, NEONICOTINOID INSECTICIDE RESIDUES, NICOTINIC ACETYLCHOLINE-RECEPTOR, COMET ASSAY, IN-VITRO, OXIDATIVE STRESS, REPRODUCTIVE TOXICITY, INDUCED NEUROTOXICITY, SUBCHRONIC EXPOSURE, ANTIOXIDANT ENZYMES, LIPID-PEROXIDATION
  • Anadolu Üniversitesi Adresli: Evet

Özet

Neonicotinoids, which are widely used worldwide, including in Turkey, are an insecticide group that are synthetic derivatives of nicotine. Recently, they have attracted attention due to their toxic effects on non-target organisms, especially bees. Numerous studies have shown that neonicotinoids have been found in detectable levels in the environment and cause various undesirable effects on living organisms, including humans and other mammals. In this study, the possible toxic effects of imidacloprid and acetamiprid, commonly used neonicotinoids, are investigated by their pure forms and commercial formulations on HT-29 cells with individual and combined exposures. According to our results, imidacloprid and acetamiprid induced cytotoxicity by caspasemediated apoptosis, mitochondrial membrane depolarization, DNA damage, and oxidative stress under these experimental conditions. It is worth mentioning low doses of DNA damage, mixture exposure causes toxic effects at lower concentrations than individual exposure, and formulation groups are at the forefront of toxicity formation, though this varies depending on the parameters.