Extraction of poppy seed oil with supercritical carbon dioxide (SC-CO2) was performed and the effect of extraction conditions on oil solubility and yield as well as oil composition was evaluated. Within the temperature (50 to 70degreesC) and pressure (21 to 55 MPa) ranges studied, 55 MPa/70degreesC gave the highest on solubility (24.1 mg oil/g CO2) and oil yield (38.7 g oil/100g seed). Fatty acid composition of the oil obtained with SC-CO2 at 55 MPa/70degreesC was similar to that of petroleum ether-exitracted oil (p > 0.05) with linoleic acid making up 69.0 to 73.7% of fatty acids. Tocol content of the SC-CO2-extracted oils varied from 2237 to 33.35 mg/100 g oil, which was higher than that of petroleum ether-extracted oil (15.28 mg/100 g oil). Poppy seed oil may have potential in the rapidly growing specialty oil market.